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All of the following statements are true about restriction enzymes EXCEPT


A) they are made by bacteria and viruses.
B) hundreds of different ones have been isolated and purified.
C) they produce single-stranded complementary ends that can join to two different DNA strands by complementary base pairing.
D) each enzyme cuts DNA at a different specific base sequence.

E) All of the above
F) B) and C)

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A

State the difference between a transgenic animal and a cloned animal.

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Transgenic organisms are also called gen...

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Plants are being genetically engineered to have


A) a requirement for more fertilizer.
B) an increased water requirement.
C) the ability to produce human proteins.
D) increased susceptibility to herbicides.
E) All of the choices are correct.

F) B) and D)
G) A) and E)

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What is the function of naturally occurring restriction enzymes in bacterial cells?


A) They are used during DNA replication in the bacterial cell.
B) They are used to degrade the bacterial cell's DNA.
C) Restriction enzymes recognize and cleave DNA molecules that are foreign to the bacterial cell.
D) These enzymes are used to attach pieces of DNA into an opening created by ligase enzymes.

E) C) and D)
F) A) and B)

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Transgenic _______ have been given suicide genes that cause them to self-destruct when the job for which they are engineered has been accomplished.


A) animals
B) bacteria
C) plants
D) fungi
E) All of the choices are correct.

F) D) and E)
G) B) and D)

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Describe the newest method of DNA fingerprinting, using fluorescent STR profiling.

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Short tandem repeat (STR) profiling is c...

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Proteomics involves the application of computer technologies to study databases of genetic information.

A) True
B) False

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Under which of the following circumstances would a DNA microarray be useful?


A) It would enable a researcher to determine the differences in gene expression between cell types.
B) Researchers are trying to determine if an individual has inherited a genetic disease from their parents.
C) It would enable doctors to determine which genes are responsible for producing diseased tissue in an individual.
D) All of these are circumstances in which a DNA microarray would be useful.
E) None of these are circumstances in which a DNA microarray would be useful.

F) A) and E)
G) A) and B)

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Human gene therapy


A) includes the insertion of genetic material into human cells for the treatment of a genetic disease.
B) has been used for treatment of children who have severe combined immunodeficiency.
C) has been used in a trial to treat familial hypercholesterolemia.
D) All of the choices are correct.

E) B) and D)
F) A) and B)

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What was the minimal requirement to perform a "classic DNA fingerprinting" test in order to associate a blood sample with a criminal?


A) one sample of DNA, PCR amplification, restriction enzymes, and gel electrophoresis
B) one sample of DNA, restriction enzymes, a DNA synthesizer, and gel electrophoresis
C) two samples of DNA, PCR amplification, restriction enzymes, and gel electrophoresis
D) two samples of DNA, PCR amplification, a DNA synthesizer, and gel electrophoresis
E) two samples of DNA, PCR amplification, restriction enzymes, and a DNA synthesizer

F) D) and E)
G) B) and E)

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How are DNA microarrays used in the study of genomics?


A) They are used to identify the mutations that an individual possesses.
B) They can be used to determine which genes are turned on or off in an individual.
C) They can be used to help determine an individuals genetic profile.
D) All of these are uses for DNA microarrays.
E) None of these are uses for DNA microarrays.

F) All of the above
G) C) and D)

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Small, variable regions of DNA that may change an individual's susceptibility to disease are called proteomes.

A) True
B) False

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What is the difference between ex vivo and in vivo gene therapy?

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In ex vivo gene therapy, tissue such as ...

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Biotechnology products produced by bacteria include all of the following EXCEPT


A) an antibody to deliver radioisotopes to tumor cells.
B) clotting factor VIII.
C) human growth hormone.
D) insulin.

E) All of the above
F) A) and D)

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The polymerase chain reaction uses RNA polymerase.

A) True
B) False

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Which of the following molecules forms lengths of DNA with "sticky ends"?


A) DNA ligase
B) DNA polymerase
C) RNA polymerase
D) reverse transcriptase
E) restriction enzyme

F) B) and D)
G) A) and E)

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E

Explain the applications for polymerase chain reaction technology.

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Answers will vary but should include the...

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All of the following statements about CNVs are true EXCEPT


A) CNVs occur when genes have changed their number.
B) individuals may differ as to the number of copies of genes.
C) CNVs have known links to disease.
D) All of the answer choices are true.

E) A) and B)
F) A) and D)

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D

Proteomics is the study of the exact role of the genome in cells or organisms.

A) True
B) False

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All of the following are true regarding transgenic animals EXCEPT


A) foreign genes are micro-injected into eggs, fertilized in vitro, and the zygotes are placed in host females to develop.
B) the process has created mice that have a phenotype similar to that of a person with cystic fibrosis.
C) the process has been used to develop organisms that are partially animals and partially plant.
D) the product desired by the transgenic procedure may be secreted in the milk of female offspring.

E) A) and C)
F) B) and C)

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